IL-4 anticorps

N° du produit ABIN118404

Cet anticorps anti-IL-4 est un anticorps Rat Monoclonal détectant IL-4 dans WB, FACS, EIA, IHC (fro) et ELISA (Capture). Adapté pour Souris.

Aperçu rapide pour IL-4 anticorps (ABIN118404)

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-4 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA (Capture)

Clone: BVD4-1D11

Détail du produit anti-IL-4 anticorps

Specificité: This antibody is specific for Mouse Interleukin-4 (IL-4). Clone BVD4-1D11 is reported to neutralise the effects of IL-4. We recommend the use of SM101A for functional studies.

Aucune reactivité croisée: Rat (Rattus)

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on protein G

Immunogène: Recombinant Mouse IL-4. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required. Immunohistochemistry on frozen sections. ELISA: 1 μg/mL - 5 μg/mL. Western Blot: 1 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur IL-4

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Autre désignation: Interleukin-4 / IL4

Sujet: Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra

ID gène: 16189

NCBI Accession: NP_067258

UniProt: P07750

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation